BMP-5 BioAssay™ ELISA Kit (Bone Morphogenetic Protein 5) (Canine)

Katalog-Nummer 396580-96T

Size : 96Tests

Marke : US Biological

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396580 BMP-5 BioAssay™ ELISA Kit (Bone Morphogenetic Protein 5) (Canine)

Clone Type
Polyclonal
Swiss Prot
P22003
Applications
E
Shipping Temp
Blue Ice
Storage Temp
4°C/-20°C

Bone morphogenetic protein 5 is a protein that in Canine as is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's, BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on Canine a chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18kD.||Sample Type:|Cell culture supernates, serum, bone tissue, tissue.||Intended Use:|Sandwich High Sensitivity ELISA kit for Quantitative Detection of Canine BMP-5. ||Sensitivity:|<10pg/ml||Range:|156pg/ml-10,000g/ml||Specificity:|Recognizes natural and recombinant canine BMP-5. There is no detectable cross-reactivity with other relevant proteins.||Test Principle:|This Canine BMP-5 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Canine BMP-5 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: A317-H454) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Canine BMP-5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Canine BMP-5 amount of sample captured in plate.||Kit Components:|96-well plate precoated with anti-Canine BMP-5 antibody, 1x plate|Lyophilized recombinant Canine BMP-5 standard, 2x10ng/tube|Biotinylated anti-Canine BMP-5 antibody, 1x130ul (dilution 1:100)|Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)|Sample diluent buffer, 1x30ml|Antibody diluent buffer, 1x12ml|ABC diluent buffer, 1x12ml|TMB color developing agent, 1x10ml|TMB stop solution, 1x10ml|PBS washing buffer Powder for 1000ml, 1x buffer||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.
References
1. Hahn GV, Cohen RB, Wozney JM, Levitz CL, Shore EM, Zasloff MA, Kaplan FS (Dec 1992). "A bone morphogenetic protein subfamily: chromosomal localization of human genes for BMP5, BMP6, and BMP7". Genomics 14 (3): 759–62. |2. Beck HN, Drahushuk K, Jacoby DB, Higgins D, Lein PJ (Mar 2003). "Bone morphogenetic protein-5 (BMP-5) promotes dendritic growth in cultured sympathetic neurons". BMC Neurosci 2: 12.|3. "Entrez Gene: BMP5 bone morphogenetic protein 5". |4. Hahn, G. V., Cohen, R. B., Wozney, J. M., Levitz, C. L., Shore, E. M., Zasloff, M. A., Kaplan, F. S.A bone morphogenetic protein subfamily: chromosomal localization of human genes for BMP5, BMP6, and BMP7.Genomics14: 759-762, 1992.