Catenin alpha1 (CTNNA1, Catenin (Cadherin-associated Protein), alpha 1, 102kDa, HGNC:2509, CAP102, FLJ36832, alpha 1 (102kD), alpha-E-Catenin, alpha-Catenin, alphaE-Catenin) (FITC)

Katalog-Nummer C2069-43-FITC-100ul

Size : 100ul

Marke : US Biological

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C2069-43-FITC Catenin alpha1 (CTNNA1, Catenin (Cadherin-associated Protein), alpha 1, 102kDa, HGNC:2509, CAP102, FLJ36832, alpha 1 (102kD), alpha-E-Catenin, alpha-Catenin, alphaE-Catenin) (FITC)

Clone Type
Polyclonal
Host
mouse
Source
mouse
Isotype
IgG1,k
Grade
Affinity Purified
Applications
E IF IHC IP WB
Crossreactivity
Ch Hu Mo Rt Xe
Shipping Temp
Blue Ice
Storage Temp
-20°C

a-catenin is a 102kD protein which is related to the cytoskeletal protein vinculin. Vinculin associates with both cell-cell and cell-extracellular matrix adherens-type junctions, and has been shown to interact with both a-actinin and actin. The homology between a-catenin and vinculin is localized to three major regions (amino-terminal, central, and carboxy-terminal portions) with the c-termini exhibiting the highest overall homology . Interestingly, it is the carboxy terminal domain of vinculin which is responsible for its self-association properties . The homology between the c-termini of catenin and vinculin led to speculation that a-catenin can self-associate and/or co-associate with vinculin thereby linking the cadherin/catenin complex to the cytoskeleton. However, recent studies indicate that cadherin/catenin complexes are linked to the actin cytoskeleton via a direct association between a-actinin and a-catenin. In addition, the a-catenin protein has been found to posses both actin binding and bundling activities.||Applications: |Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Immunofluorescence: 2-5ug/ml|ELISA: 0.1-1ug/ml |Western Blot: 1ug/ml |Immunohistochemistry ( formalin-fixed, paraffin-embedded tissue): 2-10ug/ml. Heat-Induced Epitope Retrieval (HIER) is required. Expected staining pattern: Membrane with limited cytoplasmic.|Immunoprecipitation: 5ug|Optimal dilutions to be determined by the researcher.||Positive Control: |Pancreas/breast tumor/heart ||Source: |Ascites ||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. |Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1,k|Clone No: 3G59 (7A4)|Host: mouse|Source: mouse|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).|Purity: Purified by immunoaffinity chromatography.|Immunogen: Synthetic peptide derived from the C-terminus of mouse a-catenin.|Specificity: Recognizes mouse a-catenin. Does not crossreact with the related beta- and gamma-catenin proteins. Species Crossreactivity: Human, rat, chicken, Xenopus. Lysates Tested: HeLa cells, A431 cells, WI-38 human fibroblasts, Xenopus cytosol, NIH 3T3 cells.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic peptide derived from the C-terminus of mouse a-catenin.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse a-catenin. Does not crossreact with the related beta- and gamma-catenin proteins. Species Crossreactivity: Human, rat, chicken, Xenopus. Lysates Tested: HeLa cells, A431 cells, WI-38 human fibroblasts, Xenopus cytosol, NIH 3T3 cells.