CD117 (c-kit, Stem Cell Factor, SCF, Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF) (APC)

Katalog-Nummer C2448-09D-APC-100ul

Size : 100ul

Marke : US Biological

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C2448-09D-APC CD117 (c-kit, Stem Cell Factor, SCF, Receptor, Steel Factor Receptor, Mast Cell Growth Factor, MGF) (APC) discontinued

Clone Type
Polyclonal
Host
mouse
Source
human
Swiss Prot
P10721
Isotype
IgG1
Grade
Affinity Purified
Applications
FC FLISA
Crossreactivity
Bo Hu Mk
Accession #
NP_000213.1
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

CD117 is expressed by some haematopoietic stem cells (approx. 70% of CD34+ve cells in normal bone marrow are CD117+ve), by some acute myeloid leukemias and by mast cells. Functionally CD117 is a receptor for stem cell factor and has receptor tyrosine kinase activity. This antibody is non-neutralizing. ||Applications:|Suitable for use in FLISA and Flow Cytometry. Other applications have not been tested.||Recommended Dilutions:|Optimal working dilutions to be determined by researcher.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 104D2|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by affinity chromatography.|Immunogen: MOLM-1 megakaryocyte cell line.|Specificity: Recognizes the human CD117 cell surface antigen, a 145kD glycoprotein also known as c-kit. Species Crossreactivity: Cynomolgus monkey and bovine.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
MOLM-1 megakaryocyte cell line.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by affinity chromatography.
Specificity
Recognizes the human CD117 cell surface antigen, a 145kD glycoprotein also known as c-kit. Species Crossreactivity: Cynomolgus monkey and bovine.
References
1. Broudy, V.C. et al. (1998). Analysis of c-kit receptor dimerization by fluorescence resonance energy transfer. Blood 91: 898-906.2. Yoshino, N. et al. (2000) Exp. Anim. 49:97-110