CD133 (AC133, Antigen AC133, CD133, CD133 Antigen, CD133 Retinal Degeneration, CORD12, MCDR2, MSTP061, Prominin 1, Prominin-1, PROM1, PROM, Prominin-like Protein 1, PROML1, RP41, STGD4) (APC)

Katalog-Nummer C2514-90J-100ug

Size : 100ug

Marke : US Biological

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C2514-90J CD133 (AC133, Antigen AC133, CD133, CD133 Antigen, CD133 Retinal Degeneration, CORD12, MCDR2, MSTP061, Prominin 1, Prominin-1, PROM1, PROM, Prominin-like Protein 1, PROML1, RP41, STGD4) (APC)

Clone Type
Polyclonal
Host
rat
Source
mouse
Isotype
IgG2a
Grade
Affinity Purified
Applications
FC
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

CD133 (also known as Prominin-1 or AC133) is a 120 kD pentaspan glycoprotein with 5 transmembrane domains which localize to cellular protrusions. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to hematopoietic stem cells, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. It also has been detected at low levels in the kidney, pancreas, placenta, and fetal liver tissue. Although the biological function of CD133 is not conclusive, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.||Applications:|Suitable for use in Flow Cytometry. Other applications have not been tested. ||Recommended Dilution:|Flow Cytometry: ≤0.25ug/10e6 cells in 100ul volume|Optimal dilutions to be determined by researcher. ||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: 315-2C11|Host: rat|Source: mouse|Concentration: ~0.2mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Allophycocyanin (APC) |Purity: Purified by affinity chromatography.|Immunogen: CD133-expressing-GFP-RBL2H3 plus CFA as adjuvant.|Specificity: Recognizes mouse CD133.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
CD133-expressing-GFP-RBL2H3 plus CFA as adjuvant.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Allophycocyanin (APC)
Purity
Purified by affinity chromatography.
Specificity
Recognizes mouse CD133.
References
1. Singh SK, et al. 2004. Nature 18:396. 2. Falini B, et al. 2005. New Engl. J. Med. 352:254. 3. Bonanno G, et al. 2004. Transfusion 44:1087. 4. Gaipa G, et al. 2002. Haematologica 87:347. 5. Wuchter C, et al. 2001. Haematologica 86:154. 6. Zhang Y, et al. 2012. Diabetes. 61:2114.