CD27 (SWC2, Swine Workshop Cluster 2) (APC)

Katalog-Nummer 397010-100T

Size : 100Tests

Marke : US Biological

Weitere Informationen anfordern

Contact local distributor :


Telefonnummer : +1 850 650 7790


397010 CD27 (SWC2, Swine Workshop Cluster 2) (APC)

Clone Type
Polyclonal
Host
mouse
Source
porcine
Swiss Prot
F1SL30
Isotype
IgG1
Grade
Affinity Purified
Applications
FC
Crossreactivity
Po
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

In humans the CD27 antigen is expressed by discrete populations of T- and B-cells where it functions in a co-stimulatory role to induce proliferation of T-cells and B-cells, however studies undertaken using the CD27 have been unable to detect expression of CD27 on porcine B-cells. Studies in humans have demonstrated CD27 to be involved in apoptosis, research in swine has shown that porcine CD27 is expressed by all naïve CD8a-ve T-helper cells and a sub-population of CD8a+ve cells. Monoclonal antibodies to CD27 have previously been used to differentiate between subsets of NK cells and research utilizing CD27 has shown that this monoclonal antibody may be used to differentiate between subsets of porcine NK cells.||Applications: |Suitable for use in Flow Cytometry. Other applications not tested.||Recommended Dilution:|Flow Cytometry: Neat |Optimal dilutions to be determined by the researcher.||Hybridoma:|SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: B30C7|Host: mouse|Source: porcine|Concentration: Not determined|Form: Supplied as a lyophilized powder in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml dH2O. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein A affinity chromatography from tissue culture supernatant.|Immunogen: Porcine peripheral blood monocytes.|Specificity: Recognizes porcine CD27.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Porcine peripheral blood monocytes.
Form
Supplied as a lyophilized powder in PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml dH2O. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes porcine CD27.
References
1. Reutner, K. et al. (2012) Porcine CD27: identification, expression and functional aspects in lymphocyte subsets in swine. Dev Comp Immunol. 38: 321-31.|2. Reutner, K. et al. (2013) CD27 expression discriminates porcine T helper cells with functionally distinct properties. Vet Res. 44: 18.|3. Franzoni, G. et al. (2013) Assessment of the Phenotype and Functionality of Porcine CD8 T Cell Responses following Vaccination with Live Attenuated Classical Swine Fever Virus (CSFV) and Virulent CSFV Challenge. Clin Vaccine Immunol. 20: 1604-16.|4. Mair, K.H. et al. (2013) Porcine CD8alphadim/-NKp46high NK cells are in a highly activated state. Vet Res. 44: 13.