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> CD45.1 antibody [A20] (APC-Cy7)

CD45.1 antibody [A20] (APC-Cy7)

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Katalog-Nummer GTX01463-15-100ug

Size : 100ug

Marke : GeneTex

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Anti-CD45.1 antibody [A20] (APC-Cy7) used in Flow cytometry (FACS). GTX01463-15

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 A20

Isotype

 IgG2a

Application

 FACS

Reactivity

 Mouse
Package
100 μg
Datasheet File
View product citations for antibody GTX01463-15 on CiteAb
PRODUCT

Summary

The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors.The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin-Cyanine7 (APC-Cy7) Wavelength

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

DATA IMAGES

Anti-CD45.1 antibody [A20] (APC-Cy7) used in Flow cytometry (FACS). GTX01463-15

GTX01463-15 FACS Image

FACS analysis of mouse SJL splenocytes using GTX01463-15 CD45.1 antibody [A20] (APC-Cy7).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 1 μg (5 μl)

Clone Reference

2012; ZBTB1 is a determinant of lymphoid development.
2012; Canonical autophagy dependent on the class III phosphoinositide-3 kinase Vps34 is required for naive T-cell homeostasis.
2010; Memory T cells persisting within the brain after local infection show functional adaptations to their tissue of residence.
2004; Localization of marginal zone macrophages is regulated by C-C chemokine ligands 21/19.

REVIEW

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Clone Reference
2012; ZBTB1 is a determinant of lymphoid development.
2012; Canonical autophagy dependent on the class III phosphoinositide-3 kinase Vps34 is required for naive T-cell homeostasis.
2010; Memory T cells persisting within the brain after local infection show functional adaptations to their tissue of residence.
2004; Localization of marginal zone macrophages is regulated by C-C chemokine ligands 21/19.
SDS
Sodium Azide.pdf