CD9 Mouse Monoclonal Antibody (HI9a), R-PE Conjugate - Biotium Choice
Katalog-Nummer P003S-RPE-125
Size : 125UL
Marke : Biotium
Contact local distributor :
Telefonnummer : +1 850 650 7790
| Antibody number | #P003 |
|---|---|
| SwissProt | P21926 |
| Biotium Choice Antibody | Primary |
| Antibody type | Biotium Choice Flow Antibodies, Primary |
| Clonality | Monoclonal |
| Host species | Mouse |
| Clone | HI9a |
| Isotype | IgG1, kappa |
| Antibody reactivity (target) | CD9 |
| Synonyms | Tetraspanin-29 (TSPAN29); BA-2/p24 antigen; BA2; BTCC1; CD9; Cell growth-inhibiting gene 2 protein; DRAP27; GIG2; Leukocyte antigen MIC3; MIC3; Motility-related protein (MRP1); p25 |
| Species reactivity | Baboon, Bovine, Cynomolgus monkey, Dog, Horse, Human, Non-human primates, Rabbit, Sheep |
| Human gene symbol | CD9 |
| Entrez gene ID | 928 |
| Unigene | 114286 |
| Molecular weight | 25 kDa |
| Antibody target cellular localization | Exosomes/EVs, Plasma membrane |
| Cell/tissue expression | Exosomes, Platelets, Basophils, Eosinophils, Epithelial cells, Lymphocytes |
| Verified antibody applications | Flow (surface) (verified), WB (verified), IF (verified) |
| Positive control | Human peripheral blood platelets, BT-20, MCF-7, HeLa, or ZR-75 cells. Cervix or esophagus tissue. |
| Antibody application notes | Flow Cytometry: 0.5-1 ug/mL (or, 0.05-0.1 ug/million cells), Immunofluorescence: 1 ug/mL, Western Blot: 0.05 ug/mL |
| Antibody research areas | Exosomes/EVs |
| Antibody/conjugate formulation | Purified: 0.5 mg/mL in PBS/0.05% azide, Conjugates: PBS/0.1% BSA/0.05% azide |
| Shelf life | Guaranteed for at least 24 months from date of receipt when stored as recommended |
| Storage Conditions | Store at 2 to 8 °C |
| Volume per assay | 5 uL/test |
| Regulatory status | For research use only (RUO) |
| Product origin | Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot. |
Product Description
Note: CD9 clone HI9a is being discontinued and has been replaced by
CD9 Recombinant Monoclonal Mouse Antibody (2310.9) – Biotium Choice
, which has been validated to have the same bright and specific staining of human CD9.CD9 (clone HI9a) is a mouse monoclonal antibody that recognizes the human tetraspanin protein CD9. This antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance. This antibody has been validated in flow cytometry, in immunofluorescence microscopy, and in western blot. The antibody is available conjugated to R-PE or Astral™ tandem dyes. They are supplied in PBS, 0.1% BSA, 0.05% azide.
CD9 is a type IV transmembrane glycoprotein with four transmembrane domains. CD9 on pre-B cells may play a role in cell-cell adhesion. In addition, CD9 may play a role in signal transduction mediated by interaction with low molecular weight GTP binding proteins. CD9 is expressed on early B cells, eosinophils, basophils and activated T cells and is a major component of the platelet cell surface. It is also expressed on most non-T acute lymphoblastic leukemia cells and on some acute myeloid and chronic lymphoid leukemia.
Tetraspanin proteins CD9, CD63, and CD81, are the most common proteins used as exosome markers. Tetraspanins are plasma membrane proteins with many proposed functions, including activation and sorting of other membrane proteins. They are also thought to play a role in the targeting of proteins to multivesicular bodies (MVBs) and exosomes. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of exosomes, but their expression levels vary depending on the cell type of origin.
As an isotype control, we recommend
Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331)
for this antibody.View our full catalog of Biotium Choice antibodies
, optimized and validated for the best results in flow cytometry.Exosome Stains and Antibodies
Biotium also offers
ExoBrite™ CD9 Flow Antibody
andExoBrite™ CD9 Western Antibody
that are validated for optimal detection of isolated exosomes by flow cytometry or western blotting.Learn more about our products for exosome research
.CD9 Monoclonal Mouse Antibody (HI9a) - Biotium Choice
| Conjugation | Ex/Em | Conc. | Size | Catalog No. | Availability |
|---|---|---|---|---|---|
| CF®405M | 416/452 nm | 100 ug/mL | 25 tests (125 uL) | P003S-405M-125 | Discontinued, see replacement P015-405M |
| 100 tests (500 uL) | P003S-405M-500 | ||||
| CF®488A | 490/516 nm | 100 ug/mL | 25 tests (125 uL) | P003S-488A-125 | Discontinued, see replacement P015-488A |
| 100 tests (500 uL) | P003S-488A-500 | ||||
| CF®568 | 562/584 nm | 100 ug/mL | 25 tests (125 uL) | P003S-568-125 | Discontinued, see replacement P015-568 |
| 100 tests (500 uL) | P003S-568-500 | ||||
| RPE-Astral™616 | 496, 546, 566/617 nm | 100 ug/mL | 25 tests (125 uL) | P003S-R616-125 | Available |
| 100 tests (500 uL) | P003S-R616-500 | ||||
| CF®647Plus | 652/668 nm | 100 ug/mL | 25 tests (125 uL) | P003S-647P-125 | Discontinued, see replacement P015-647P |
| 100 tests (500 uL) | P003S-647P-500 | ||||
| CF®700 | 696/721 nm | 100 ug/mL | 25 tests (125 uL) | P003S-700-125 | Discontinued, see replacement P015-700 |
| 100 tests (500 uL) | P003S-700-500 | ||||
APC-Astral™813 | 633, 638/813 nm | 100 ug/mL | 25 tests (125 uL) | P003S-A813-125 | Available |
| 100 tests (500 uL) | P003S-A813-500 | ||||
R-PE | 496, 546, 565/576 nm | 50 ug/mL | 25 tests (125 uL) | P003S-RPE-125 | Available |
| 100 tests (500 uL) | P003S-RPE-500 | ||||
| Purified (BSA-free) | N/A | 0.5 mg/mL | 200 uL (100 ug) | P003-200uL | Discontinued, see replacement P015 |