CF®488A Monoclonal Mouse Anti-Fluorescein (FITC) IgG, 2 mg/mL
Katalog-Nummer 20210-1
Size : 50uL
Marke : Biotium
Contact local distributor :
Telefonnummer : +1 850 650 7790
Fluorescein (FITC) Monoclonal Mouse Antibody (1F8.1E4)
Monoclonal mouse anti-fluorescein (FITC) antibody labeled with our superior CF® dyes.
Technology
CF® Dyes
Considerations for Immunofluorescence Staining
Secondary Antibody Product Listings
Tech Tips
Protocol: Fluorescent Western Blotting
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host
Product Description
This is a monoclonal mouse anti-fluorescein (FITC) antibody labeled with our superior CF® dyes.
- Available in 6 bright and photostable CF® dyes
- Suitable for western, immunofluorescence, and immunohistology in FFPE tissues
See our full selection of anti-tag and anti-hapten antibody conjugates.
Note: Conjugates of blue fluorescent dyes like CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Monoclonal Mouse Anti-Fluorescein (FITC)
| Conjugation | Ex/Em | Size | Catalog No. |
|---|---|---|---|
| CF®405M | 408/452 nm | 50 uL (100 ug) | 20214-1 |
| 0.25 mL (500 ug) | 20214 | ||
| CF®488A | 490/515 nm | 50 uL (100 ug) | 20210-1 |
| 0.25 mL (500 ug) | 20210 | ||
| CF®594 | 593/614 nm | 50 uL (100 ug) | 20211-1 |
| 0.25 mL (500 ug) | 20211 | ||
| CF®633 | 630/650 nm | 50 uL (100 ug) | 20212-1 |
| 0.25 mL (500 ug) | 20212 | ||
| CF®640R | 642/662 nm | 50 uL (100 ug) | 20213-1 |
| 0.25 mL (500 ug) | 20213 | ||
| CF®660R | 663/682 nm | 50 uL (100 ug) | 20399-50uL |
| 0.25 mL (500 ug) | 20399-0.25mL |
View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.
CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.
Storage and Handling
Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.
Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:
CF® Dye and biotin conjugates: add 0.5 mL dH2O
HRP or DNP conjugates: add 1 mL dH2O
Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.
Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.
Note: Storage of the antibody for more than a day at final working dilution is not recommended.
CF is a registered trademark of Biotium, Inc.
References
Download a list of CF® dye references.