CRP ELISA Kit (Monkey)

Katalog-Nummer OKWB00291

Size : 96Wells

Marke : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for CRP ELISA Kit (Monkey) (OKWB00291)
Product Info
Predicted Species ReactivityMacaca fascicularis|Monkey
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)89-104%87-105%85-96%88-101%
EDTA Plasma (n=5)84-100%90-99%86-94%82-97%
Heparin Plasma (n=5)80-99%81-93%83-93%81-98%
ELISA Kit PrincipleAviva Systems Biology Crp ELISA Kit (Monkey) (OKWB00291) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Crp has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Crp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Crp captured in the well.
ELISA Kit Range1.563-100ng/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)93-10497
EDTA Plasma (n=5)90-10598
Heparin Plasma (n=5)88-9591
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
Crp Microplate96 Wells (12 x 8 Well strips)
Crp Lyophilized Standard2 x 40 ng
100X Crp Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity0.375 ng/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolCRP
Alias SymbolsCRP
NCBI Gene Id103223751
Protein NameC-reactive protein
Uniprot IDA0A0D9S3Z7
Protein Accession #XP_007974764
Nucleotide Accession #XM_007976573

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