Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

Cytokeratin 7 (Glandular and Transitional Epithelial Marker) (rOV-TL12/30), CF770 conjugate, 0.1mg/mL

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Katalog-Nummer BNC701841-100

Size : 100uL

Contact local distributor :

Telefonnummer : +1 850 650 7790

Marke : Biotium

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Contact local distributor :

Telefonnummer : +1 850 650 7790

Cytokeratin 7 Recombinant Monoclonal Mouse Antibody (rOV-TL12/30)

Product Attributes

Antibody number	#1841
Antibody reactivity (target)	Cytokeratin 7
Antibody type	Primary
Host species	Mouse
Clonality	Recombinant Monoclonal
Clone	rOV-TL12/30
Isotype	IgG1
Molecular weight	55 kDa
Synonyms	CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7
Human gene symbol	KRT7
Entrez gene ID	3855
SwissProt	P08729
Unigene	411501
Immunogen	OTN 11, ovarian carcinoma cell line
Antibody target cellular localization	Cytoskeleton
Verified antibody applications	Flow (intracellular) (verified), IHC (FFPE) (verified), WB (verified)
Species reactivity	Human
Antibody application notes	Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 0.5-1 ug/mL, Immunohistology (formalin): 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Optimal dilution for a specific application should be determined by user
Positive control	HeLa cells. Carcinoma of ovary, lung, cervix, or breast
Shipping condition	Room temperature
Storage Conditions	Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life	Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status	For research use only (RUO)
Antibody/conjugate formulation	Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Antibody research areas	Cancer, Cytoskeleton
Product origin	Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Cell/tissue expression	Epithelial cells
Tumor expression	Ovarian cancer

Technology

CF® Dyes

Considerations for Immunofluorescence Staining

Primary & Secondary Antibody Conjugates

Product Description

This antibody recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7 ) from colon carcinomas (keratin 7-).

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email info@clinisciences.com to inquire about stock status and lead times before placing your order.

Catalog number key for antibody number 1841, Anti-Cytokeratin 7 (rOV-TL12/30)

Antibody # prefix	Conjugation	Ex/Em (nm)	Laser line	Detection channel	Dye Features
BNC04	CF®405S	404/431	405	DAPI (microscopy), AF405	CF®405S Features
BNC88	CF®488A	490/515	488	GFP, FITC	CF®488A Features
BNC68	CF®568	562/583	532, 561	RFP, TRITC	CF®568 Features
BNC94	CF®594	593/614	561	Texas Red®	CF®594 Features
BNC40	CF®640R	642/662	633-640	Cy®5	CF®640R Features
BNC47	CF®647	650/665	633-640	Cy®5	CF®647 Features
BNC74	CF®740	742/767	633-685	775/50	CF®740 Features
BNCB	Biotin	N/A	N/A	N/A
BNUB	Purified	N/A	N/A	N/A
BNUM	Purified, BSA-free	N/A	N/A	N/A

Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.