Reagenzien und Instrumente für die Immunologie Zellbiologie und Molekularbiologie

DA(Dopamine) ELISA Kit

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Katalog-Nummer E-EL-0046-96T

Size : 96T

Contact local distributor :

Telefonnummer : +1 850 650 7790

Marke : Elabscience

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Contact local distributor :

Telefonnummer : +1 850 650 7790

Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	50 μL
Total Assay Time	2 h 30 min
Reacitivity	Universal
Specificity	This kit recognizes Universal DA in samples.No significant cross-reactivity or interference between Universal DA and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal DA. During the reaction, Universal DA in the sample or standard competes with a fixed amount of Universal DA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal DA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal DA in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Background

Dopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.

Research Area

Neuroscience