Dihydroethidium [104821-25-2]
Katalog-Nummer T2021-25mg
Size : 25mg
Marke : TargetMol
Dihydroethidium
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Purity:99.52%
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COA HPLC HNMR
Dihydroethidium
Catalog No. T2021Cas No. 104821-25-2
Dihydroethidium (PD-MY 003) is a peroxide indicator with blue fluorescence in the cytoplasm (λex=518 nm, λem=616 nm), which disappears when oxidized and is cell permeable.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose. Pack Size | |
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5 mg | |
10 mg | |
25 mg | |
50 mg | |
100 mg | |
200 mg | |
500 mg | |
1 mL x 10 mM (in DMSO) |
Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
Description | Dihydroethidium (PD-MY 003) is a peroxide indicator with blue fluorescence in the cytoplasm (λex=518 nm, λem=616 nm), which disappears when oxidized and is cell permeable. |
In vitro | METHODS: Detection of cellular peroxides by Dihydroethidium: 1. Prepare a 5-10 mM stock solution of Dihydroethidium dye in DMSO, protect from light, and store in portions at -20℃. Dilute the stock solution with physiological buffers (e.g., PBS, HBSS, HEPES) to obtain a working solution of 5-20 µM Dihydroethidium dye. The optimal working concentration must be determined empirically. 3. Add the Dye Working Solution to the cells at a 1:1 ratio (100 µL of Dye Working Solution to 100 µL of Cell Suspension) and incubate for 5-60 minutes at room temperature or 37℃. 4. Wash with PBS and then perform fluorescence microscopy or flow cytometry. Dihydroethidium itself is blue fluorescent with a maximum excitation wavelength of 370 nm and a maximum emission wavelength of 420 nm, and after dehydrogenation, it binds to RNA or DNA to produce red fluorescence with a maximum excitation wavelength of 300 nm and a maximum emission wavelength of 610 nm, and 518 nm can be used as the excitation wavelength in practice. |
Alias | PD-MY 003, Hydroethidine |
Molecular Weight | 315.41 |
Formula | C21H21N3 |
Cas No. | 104821-25-2 |
Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | ||||||||||||||||||||
Solubility Information | DMSO: 5 mg/mL (15.85 mM) | ||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||
DMSO
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Calculator
In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSO
main solution, add 300 μLPEG300
mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O
mix well and clarify
Dose Conversion
You can also refer to dose conversion for different animals. More
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
InhibitorDihydroethidiumPD-MY003inhibitPD-MY-003
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