DL-Dithiothreitol (DTT, high purity)

DL-Dithiothreitol
Catalog number: B2010240
Lot number: Batch Dependent
Expiration Date: Batch dependent
Volume/Weight: 1 g
pH: na
Supplied as: Ready-to-use
Appearance: Powder
Applications: high purity reducing agent (> 99% by titration) also known as Cleland’s reagent.
Storage: -20C
Keywords: threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
Grade: Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.

References:
1: Bub CB. Dithiothreitol treatment of red blood cells. Immunohematology. 2017
Dec;33(4):170-172.

2: Lorenzen H, Lone Akhtar N, Nielsen M, Svendsen L, Andersen P. Thirty-three-
day storage of dithiothreitol-treated red blood cells used to eliminate
daratumumab interference in serological testing. Vox Sang. 2018
Oct;113(7):686-693.

3: Liang P, Canoura J, Yu H, Alkhamis O, Xiao Y. Dithiothreitol-Regulated
Coverage of Oligonucleotide-Modified Gold Nanoparticles To Achieve Optimized
Biosensor Performance. ACS Appl Mater Interfaces. 2018 Jan 31;10(4):4233-4242.

4: Han JC, Han GY. A procedure for quantitative determination of
tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and
effective than dithiothreitol. Anal Biochem. 1994 Jul;220(1):5-10.

5: Lin M, Yu JZ. Dithiothreitol (DTT) concentration effect and its implications
on the applicability of DTT assay to evaluate the oxidative potential of
atmospheric aerosol samples. Environ Pollut. 2019 Aug;251:938-944.

6: Heidari R, Ghanbarinejad V, Mohammadi H, Ahmadi A, Esfandiari A, Azarpira N,
Niknahad H. Dithiothreitol supplementation mitigates hepatic and renal injury in
bile duct ligated mice: Potential application in the treatment of cholestasis-
associated complications. Biomed Pharmacother. 2018 Mar;99:1022-1032.

7: Lopes de Almeida JP, Saldanha C. Dithiothreitol revisited in red cells: a new
head for an old hat. Clin Hemorheol Microcirc. 2010;46(1):51-6. d

8: Zhang N, Dong B, Kong X, Wang C, Song W, Lin W. Two-photon imaging of
1,4-dithiothreitol (DTT) by a red-emissive fluorescent probe in living cells,
tissues and animals. Spectrochim Acta A Mol Biomol Spectrosc. 2018 Dec
5;205:528-533.

9: Wang J, Lin X, Lu L, Wu Y, Zhang H, Lv Q, Liu W, Zhang Y, Zhuang S. Temporal
variation of oxidative potential of water soluble components of ambient
PM_2.5 measured by dithiothreitol (DTT) assay. Sci Total Environ. 2019
Feb 1;649:969-978.

10: Kara DA, Borzova VA, Markossian KA, Kleymenov SY, Kurganov BI. A change in
the pathway of dithiothreitol-induced aggregation of bovine serum albumin in the
presence of polyamines and arginine. Int J Biol Macromol. 2017 Nov;104(Pt
A):889-899.

11: Yu H, Wei J, Cheng Y, Subedi K, Verma V. Synergistic and Antagonistic
Interactions among the Particulate Matter Components in Generating Reactive
Oxygen Species Based on the Dithiothreitol Assay. Environ Sci Technol. 2018 Feb
20;52(4):2261-2270.

12: Youssef M, Arnesen C, Arledge C, Langley C, Sylvester D, Sikora J, Marques
MB, Long Zheng X, Williams LA 3rd. Validation and cost-effectiveness of an in-
house DL-dithiothreitol (DTT) treatment protocol for daratumumab patients in a
large tertiary care hospital provides gateway for implementation in smaller
community hospitals. Transfus Apher Sci. 2019 Apr;58(2):152-155. d

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