DOTAP (chloride) [132172-61-3]
Katalog-Nummer HY-112754A-100mg
Size : 100mg
Marke : MedChemExpress
DOTAP chloride is a useful and effective cationic lipid for transient and stable transfection DNA (plasmids, bacmids) and modified nucleic acids (antisense oligonucleotides) with out the use of helper lipid.
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DOTAP chloride Chemische Struktur
CAS. Nr. : 132172-61-3
This product is a controlled substance and not for sale in your territory.
Based on 3 publication(s) in Google Scholar
Other Forms of DOTAP chloride:
- DOTAP methylsulfate In-stock
- DOTAP mesylate In-stock
- DOTAP chloride (Excipient) Angebot einholen
Beschreibung |
DOTAP chloride is a useful and effective cationic lipid for transient and stable transfection DNA (plasmids, bacmids) and modified nucleic acids (antisense oligonucleotides) with out the use of helper lipid[1]. |
IC50 & Target |
IC50: Cationic Lipid[1] |
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In Vitro |
liposomes are reorganized by associating with DNA. The transfection efficiency of DOTAP liposomes is mainly influenced by lipid composition and cell type, but not by size or zeta potential[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Klinische Studie |
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Molekulargewicht |
698.54 |
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Formel |
C42H80ClNO4 |
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CAS. Nr. |
132172-61-3 |
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Appearance |
<35°C Solid,>38°C Liquid |
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Color |
White to off-white |
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SMILES |
CCCCCCCC/C=C\CCCCCCCC(OCC(OC(CCCCCCC/C=C\CCCCCCCC)=O)C[N+](C)(C)C)=O.[Cl-] |
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Versand | Room temperature in continental US; may vary elsewhere. |
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Speicherung |
-20°C, protect from light, stored under nitrogen *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen) |
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Lösungsmittel & Löslichkeit |
In Vitro:
Ethanol : 130 mg/mL (186.10 mM; Need ultrasonic) DMSO : 25 mg/mL (35.79 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Reinheit & Dokumentation |
Purity: ≥98.0% |
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Verweise |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO / Ethanol | 1 mM | 1.4316 mL | 7.1578 mL | 14.3156 mL | 35.7889 mL |
5 mM | 0.2863 mL | 1.4316 mL | 2.8631 mL | 7.1578 mL | |
10 mM | 0.1432 mL | 0.7158 mL | 1.4316 mL | 3.5789 mL | |
15 mM | 0.0954 mL | 0.4772 mL | 0.9544 mL | 2.3859 mL | |
20 mM | 0.0716 mL | 0.3579 mL | 0.7158 mL | 1.7894 mL | |
25 mM | 0.0573 mL | 0.2863 mL | 0.5726 mL | 1.4316 mL | |
30 mM | 0.0477 mL | 0.2386 mL | 0.4772 mL | 1.1930 mL | |
Ethanol | 40 mM | 0.0358 mL | 0.1789 mL | 0.3579 mL | 0.8947 mL |
50 mM | 0.0286 mL | 0.1432 mL | 0.2863 mL | 0.7158 mL | |
60 mM | 0.0239 mL | 0.1193 mL | 0.2386 mL | 0.5965 mL | |
80 mM | 0.0179 mL | 0.0895 mL | 0.1789 mL | 0.4474 mL | |
100 mM | 0.0143 mL | 0.0716 mL | 0.1432 mL | 0.3579 mL |
DOTAP chloride Related Classifications
- Biochemische Testreagenzien
- Drug Delivery
- Cancer
- Cancer Targeted Therapy Cancer Metabolism and Metastasis
- Metabolic Enzyme/Protease
- Liposome