DTT

Biological Activity

Dithiothreitol (DTT) is a redox reagent commonly used as a reducing agent for thiolated DNA. Dithiothreitol (DTT) is also used to reduce the disulfide bonds of proteins.

Product Citations

• 

  Cell Res. 2023 Jun;33(6):464-478.

  SCUBE2 mediates bone metastasis of luminal breast cancer by modulating immune-suppressive osteoblastic niches
  DTT purchased from AbMole

• 

  Nucleic Acids Res. 2022 Aug 12;50(14):7938-7958.

  HDAC1 and PRC2 mediate combinatorial control in SPI1/PU.1-dependent gene repression in murine erythroleukaemia
  DTT purchased from AbMole

• 

  Nat Commun. 2020 Dec 22;11(1):6438.

  MaTAR25 lncRNA regulates the Tensin1 gene to impact breast cancer progression
  DTT purchased from AbMole

• 

  J Virol. 2020 Feb 14;94(5):e01791-19.

  Targeting Kaposi's Sarcoma-Associated Herpesvirus ORF21 Tyrosine Kinase and Viral Lytic Reactivation by Tyrosine Kinase Inhibitors Approved for Clinical Use
  DTT purchased from AbMole

• 

  Exp Cell Res. 2020 Nov 1;396(1):112237.

  LRTM1 promotes the differentiation of myoblast cells by negatively regulating the FGFR1 signaling pathway
  DTT purchased from AbMole

Chemical Information

Molecular Weight	154.25
Formula	C4H10O2S2
CAS Number	3483-12-3
Solubility (25°C)	DMSO: ≥ 75 mg/mL Water: ≥ 90 mg/mL
Storage	2-8°C, dry, sealed

Conversion of different model animals based on BSA (PMID: 27057123)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Amber L Kramer, et al. Assessing the oxidative potential of PAHs in ambient PM 2.5 using the DTT consumption assay

[2] Abdul Hadi Mehmood, et al. The development of an endoplasmic reticulum-targeting fluorescent probe for the imaging of 1,4-dithiothreitol (DTT) in living cells

[3] Yiwen Wang, et al. Toxicity of Dithiothreitol (DTT) to Drosophila melanogaster

[4] Brittany C Hudson, et al. The effects of dithiothreitol (DTT) on fluorescent qPCR dyes

[5] Heng Zhou, et al. Oncolysis with DTT-205 and DTT-304 generates immunological memory in cured animals