EGFRvIII (Epidermal Growth Factor Receptor, Variant III) (GFR/2600R), Biotin conjugate, 0.1mg/mL
Katalog-Nummer BNCB2600-100
Size : 100uL
Marke : Biotium
Antibody number | #2600 |
---|---|
Antibody reactivity (target) | EGFRvIII |
Antibody type | Primary |
Host species | Rabbit |
Clonality | Recombinant Monoclonal |
Clone | GFR/2600R |
Isotype | IgG |
Molecular weight | ~145 kDa (vIII variant) |
Synonyms | Erbb1 variant III; ERBB1 variant III; HER1 variant III; Proto-oncogene c-ErbB-1 variant III; Receptor Tyrosine Protein Kinase variant III; ErbB1 variant III |
Human gene symbol | EGFR |
Entrez gene ID | 1956 |
SwissProt | P00533 |
Unigene | 488293 |
Immunogen | KLH conjugate of EGFRvIII-specific, 14-amino acid peptide from the predicted amino acid sequence at the fusion junction (LEEKKGNYVVTDHC) |
Antibody target cellular localization | Plasma membrane |
Verified antibody applications | IHC (FFPE) (verified) |
Species reactivity | Human |
Antibody application notes | Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes, Optimal dilution for a specific application should be determined by user |
Positive control | A431 cells. Placenta, Breast, Colon or Bladder Carcinoma |
Shipping condition | Room temperature |
Storage Conditions | Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C |
Shelf life | Guaranteed for at least 24 months from date of receipt when stored as recommended |
Regulatory status | For research use only (RUO) |
Antibody/conjugate formulation | Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide |
Antibody research areas | Cancer, Signal transduction |
Product origin | Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot. |
Tumor expression | Squamous cell carcinoma |
Product Description
EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the
CF® Dye Brochure
for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.info@clinisciences.com
to inquire about stock status and lead times before placing your order.Catalog number key for antibody number 2600, Anti-EGFRvIII (GFR/2600R)
Antibody # prefix | Conjugation | Ex/Em (nm) | Laser line | Detection channel | Dye Features |
---|---|---|---|---|---|
BNC04 | CF®405S | 404/431 | 405 | DAPI (microscopy), AF405 | CF®405S Features |
BNC88 | CF®488A | 490/515 | 488 | GFP, FITC | CF®488A Features |
BNC68 | CF®568 | 562/583 | 532, 561 | RFP, TRITC | CF®568 Features |
BNC94 | CF®594 | 593/614 | 561 | Texas Red® | CF®594 Features |
BNC40 | CF®640R | 642/662 | 633-640 | Cy®5 | CF®640R Features |
BNC47 | CF®647 | 650/665 | 633-640 | Cy®5 | CF®647 Features |
BNC74 | CF®740 | 742/767 | 633-685 | 775/50 | CF®740 Features |
BNCB | Biotin | N/A | N/A | N/A | |
BNUB | Purified | N/A | N/A | N/A | |
BNUM | Purified, BSA-free | N/A | N/A | N/A |