The EndoCAb healthy adults in a particular locality. It has not been established whether normal endotoxin-core antibody values vary by region, culture or race. Users should establish appropriate local statistical controls for their studies. It is advised that studies of any patient group should always be controled by studies of appropriately selected contrasting clinical groups and/or healthy individuals recruited locally. EndoCAb-well strips allows free choice of batch size for the assay. Samples and standards are incubated in microtiter wells coated with equimolar amounts of endotoxin rough-lipopolysaccharides from four Gram-negative bacterial species, each comprised of a complete inner core, but lacking complete outer core or O-specific polysaccharide chain. Anti-endotoxin-core antibodies are captured by the solid phase antigen. Peroxidase conjugated anti-human IgA antibody will bind to captured endotoxin-core antibodies. The peroxidase conjugated antibody will react with the substrate, tetramethylbenzidine (TMB). The enzyme reaction is stopped by the addition of oxalic acid. The absorbance at 450 nm is measured with a spectrophotometer. A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the EndoCAb col-md-6">

Product type

Assays

Amount

1 x 96 det.

Standard range

0.05 – 3.5 MMU/ml

Detection level

0.05 MMU/ml

Working volume

100 /05/SDS-HK504-IGM.pdf" class="link" download>

SDS - HK504-IGM

61 kb

Assay Manual

HK504-IgM

356 kb