Ep-CAM / CD326 (Rat)(GZ-20), Biotin conjugate, 0.1mg/mL

Katalog-Nummer BNCB0382-100

Size : 100uL

Marke : Biotium

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Antibody number

#0382

Antibody reactivity (target)

CD326, Ep-CAM

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

GZ-20

Isotype

IgG1, kappa

Molecular weight

40-43 kDa

Synonyms

Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TACSTD1; TROP1; Tumor-associated Calcium Signal Transducer 1; ECS-1; Epidermal Surface Antigen 1; ESA1; FLOT2; Flotillin-2; M17S1 (Membrane Component; Chromosome 17; Surface Marker-1); REG-1; Reggie-1; Reggie-2

Entrez gene ID

171577

SwissProt

O55159

Immunogen

Rat small intestine cells

Antibody target cellular localization

Plasma membrane

Species reactivity

Rat

Antibody application notes

For coating for ELISA, order Ab without BSA, Optimal dilution and staining procedure for a specific application should be determined by user, Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

Positive control

Rat small intestine or colon. Rat colon lysate.

Shipping condition

Room temperature

Storage Conditions

Note: store BSA-free antibodies at -10 to -35°C, Store at 2 to 8 °C, Protect fluorescent conjugates from light

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Product origin

Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.

Cell/tissue expression

Epithelial cells

Tumor expression

Adenocarcinoma

Download Datasheet

Product Description

Epitope of GZ-1 and GZ-20 monoclonal antibodies are different. EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on basolateral cell surface in most simple epithelia and a vast majority of carcinomas.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the

CF® Dye Brochure

for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email

info@clinisciences.com

to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 0382, Anti-CD326|Ep-CAM (GZ-20) 

Antibody # prefix Conjugation Ex/Em (nm) Laser line Detection channel Dye Features
BNC04 CF®405S 404/431 405 DAPI (microscopy), AF405

CF®405S Features

BNC88 CF®488A 490/515 488 GFP, FITC

CF®488A Features

BNC68 CF®568 562/583 532, 561 RFP, TRITC

CF®568 Features

BNC94 CF®594 593/614 561 Texas Red®

CF®594 Features

BNC40 CF®640R 642/662 633-640 Cy®5

CF®640R Features

BNC47 CF®647 650/665 633-640 Cy®5

CF®647 Features

BNC74 CF®740 742/767 633-685 775/50

CF®740 Features

BNCB Biotin N/A N/A N/A
BNUB Purified N/A N/A N/A
BNUM Purified,
BSA-free
N/A N/A N/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

References

Tsubura A et. al. J Cut Pathol, 1992, 19:73-9. |Litvinov SV et. al. J Cell Biol, 1994, 125:437-46. |