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EverBrite™ Hardset Mounting Medium
A revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes. EverBrite™ Hardset mounting medium hardens at room temperature completely in 24 hours.
Product Attributes
Format
Without DAPI, With DAPI
For live or fixed cells
For fixed cells/tissue sections
Storage Conditions
Store at 2 to 8 °C, Protect from light
Probe cellular localization
Nucleus
Technology
Considerations for Immunofluorescence Staining
Antibody & Protein Labeling Kits
Tyramide Signal Amplification
Tech Tips
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst
Biotium’s EverBrite™ line of mounting media are a revolutionary antifade mounting media optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes. EverBrite™ Hardset mounting medium hardens at room temperature completely in 24 hours with excellent protection against photobleaching of fluorescent dyes. Unlike VectaShield®, EverBrite™ is compatible with cyanine-based fluorophores such as Cy®3 and Cy®5, Alexa Fluor® 555, Alexa Fluor® 647, and DyLight® 649.
Antifade mounting medium optimally formulated to prevent photobleaching of fluorophores
Hardens completely at room temperature in 24 hours
Refractive index is well-matched to that of coverslip glass and immersion oil
Cures to form a hard, permanent seal with the coverslip
No need to seal coverslip edges with nail polish or other sealants
Available with or without DAPI
EverBrite™ Hardset mounting medium cures to form a hard, permanent seal with the coverslip at room temperature in 24 hours. This facilitates handling of the slide and eliminates the need to seal coverslip edges with nail polish or other sealants. It is optimally formulated for use with Biotum’s CF® dye conjugates but also provides a high level of protection from rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes. Mounted slides can be stored at 4°C for a year or longer. EverBrite™ Hardset Mounting Medium has a refractive index of 1.38 initially, which increases to 1.42 after 24 hours of curing, and 1.46 after four days of curing, after which it remains constant. EverBrite™ Hardset Mounting Medium is available with or without DAPI for nuclear counterstaining. Mounting media with DAPI gives a blue fluorescent nuclear counterstain without an additional staining step.
We also offer EverBrite™ Mounting Media in wet-set formulation, with or without DAPI. Also see our NucSpot® Nuclear Stains and our other accessory products for cell biology research.
EverBrite™ Mounting Media
Product
Counterstain
Cat. No.
EverBrite™ Mounting Medium
None
23001
EverBrite™ Mounting Medium with DAPI
DAPI
23002
Drop-n-Stain EverBrite™ Mounting Medium
None
23008
Drop-n-Stain EverBrite™ Mounting Medium with DAPI
DAPI
23009
EverBrite™ Hardset Mounting Medium
None
23003
EverBrite™ Hardset Mounting Medium with DAPI
DAPI
23004
EverBrite™ Hardset with NucSpot® 640
NucSpot® 640
23016
EverBrite TrueBlack® Hardset Mounting Medium
None
23017
EverBrite TrueBlack® Hardset with DAPI
DAPI
23018
EverBrite TrueBlack® Hardset with NucSpot® 640
NucSpot® 640
23019
EverBrite is a trademark of Biotium, Inc.; CF is a registered trademark of Biotium, Inc.; Vectashield is a registered trademark of VectorLabs; Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific; Cy and CyDye are registered trademarks of Cytiva.