FBL ELISA Kit (Human)

Katalog-Nummer OKWB00172

Size : 96Wells

Marke : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for FBL ELISA Kit (Human) (OKWB00172)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)87-104%86-105%94-101%86-100%
EDTA Plasma (n=5)91-101%83-93%89-100%88-100%
Heparin Plasma (n=5)84-96%80-97%83-97%85-97%
ELISA Kit PrincipleAviva Systems Biology FBL ELISA Kit (Human) (OKWB00172) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FBL has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FBL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FBL captured in the well.
ELISA Kit Range1.563-100ng/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)86-10598
EDTA Plasma (n=5)87-10395
Heparin Plasma (n=5)92-9894
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
FBL Microplate96 Wells (12 x 8 Well strips)
FBL Lyophilized Standard2 x 100 ng
100X FBL Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity0.938 ng/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolFBL
Gene Full Namefibrillarin
Alias Symbols34 kDa nucleolar scleroderma antigen;34-kD nucleolar scleroderma antigen;FIB;FLRN;histone-glutamine methyltransferase;Nop1;RNA, U3 small nucleolar interacting protein 1;RNU3IP1;rRNA 2'-O-methyltransferase fibrillarin;U6 snRNA 2'-O-methyltransferase fibrillarin.
NCBI Gene Id2091
Protein NamerRNA 2'-O-methyltransferase fibrillarin
Description of TargetS-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).
Uniprot IDP22087
Protein Accession #NP_001427
Nucleotide Accession #NM_001436

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