FHC
Katalog-Nummer P0009022
Size : OneFrozenvial
Disease:
Normal
Species:
Homo sapiens, human Colon Epithelial; although FHC cells exhibit epithelial morphology, cytoplasmic keratins were not detected. Adherent 13 weeks gestation. A 1:1 mixture of DMEM:F12 Medium.To make the complete growth medium, add the following components to the base medium: extra 10 mM HEPES (for a final conc. of 25 mM); 10 ng/ml cholera toxin; 0.005 mg/ml insulin; 0.005 mg/ml transferrin; 100 ng/ml hydrocortisone; 20 ng/mL human recombinant EGF; fetal bovine serum 10% (final conc.) Remove medium, and rinse with 0.05% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Culture at 5% CO2, 37°C. A subcultivation ratio of 1:2 to 1:4 is recommended every 10-15 days. Renew medium once every 3 to 4 days depending on cell density. Complete growth medium supplemented with an extra 50% FBS and 10% (v/v) DMSO I Bacteria: Negative Yeast: Negative Mycoplasma: Negative HIV: Negative Hepatitis B: Negative Amelogenin: X,Y CSF1PO: 11,12 D13S317: 12,13 D16S539: 9,11 D5S818:12,13 D7S820: 8,12 THO1:6,9.3 TPOX: 8,11 vWA: 16 If use of this culture results in a scientific publication, it should be cited in the publication as: FHC (AddexBio P0009022) Tissue:
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References for FHC cells:
1. Siddiqui KM, Chopra DP. Primary and long term epithelial cell cultures from human fetal normal colonic mucosa. In Vitro. 1984;20(11):859-868. PMID: 6519668