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> Fibrillarin antibody [38F3]

Fibrillarin antibody [38F3]

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Katalog-Nummer GTX24566-250ul

Size : 250ul

Marke : GeneTex

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Anti-Fibrillarin antibody [38F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24566
Anti-Fibrillarin antibody [38F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24566
Anti-Fibrillarin antibody [38F3] used in Immunohistochemistry (IHC). GTX24566
Anti-Fibrillarin antibody [38F3] used in Western Blot (WB). GTX24566

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 38F3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse, Rat, Yeast, Drosophila, Caenorhabditis elegans, Schizosaccharomyces pombe, Plant
Package
250 μl
Datasheet File
View product citations for antibody GTX24566 on CiteAb

APPLICATION

Application Note

IF (ICC): 1/1000 - 1/5000 (yeast cells). For IF of mammalian cells, 1/500. WB: 1/2000 (cell lysates) - 1/10000 (nuclear fractions)(ECL). Detects a band of approximately 34 kDa. For other (non-ECL) western detection methods, 1/1000 - 1/5000. To detect mammalian fibrillarin on western blots by ECL, 1/500. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant

Preservative

10mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Yeast nuclear preparation (S. cerevisiae).Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S. cerevisiae).

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_367009

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fibrillarin , FIB , FLRN , Nop1 , RNU3IP1

Cellular Localization

Nucleolus

Background

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-Fibrillarin antibody [38F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24566

GTX24566 ICC/IF Image

Human neuroblastoma line SH-SY5Y stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red) and counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). The NF-H antibody was used at a dilution of 1/100000 and the fibrillarin monoclonal at 1/1000

Anti-Fibrillarin antibody [38F3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24566

GTX24566 ICC/IF Image

High magnification view of human Hek293 cell nuclei stained with mouse monoclonal to fibrillarin (green), counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue). Cultures were processed using our standard fixat

Anti-Fibrillarin antibody [38F3] used in Immunohistochemistry (IHC). GTX24566

GTX24566 IHC Image

Rat neurons and glial stained with mouse monoclonal to Fibrillarin (green) and with chicken antibody to neurofilament NF-H (red). Cells were counterstained with a fluorescent DNA probe (blue). Nuclear DNA is revealed with Hoechst dye (blue).

Anti-Fibrillarin antibody [38F3] used in Western Blot (WB). GTX24566

GTX24566 WB Image

Blot of yeast protein extract stained with the GTX24566 anti-Gibrillarin anitbody, stained a single band at ~34kDa.

Application Reference

Sun X et al. EMBO Rep 2023; 24 (9) : e56230 FBL promotes cancer cell resistance to DNA damage and BRCA1 transcription via YBX1.
Bennett CL et al. Heliyon 2020; 6 (6) : e04165 Tight expression regulation of senataxin, linked to motor neuron disease and ataxia, is required to avert cell-cycle block and nucleolus disassembly.
Xiangduo Kong et al. bioRxiv 2019; (Epub) The cohesin loader NIPBL interacts with pre-ribosomal RNA and treacle to regulate ribosomal RNA synthesis.
Application : ICC/IF
Reactivity : Human
Behm M et al. J Cell Sci 2017; 130 (4) : 745-753 Accumulation of nuclear ADAR2 regulates adenosine-to-inosine RNA editing during neuronal development.
Application : ICC/IF
Reactivity : Mouse
Zougman A et al. Nucleic Acids Res 2011; 39 (16) : 7103-13 Identification and characterization of a novel ubiquitous nucleolar protein 'NARR' encoded by a gene overlapping the rab34 oncogene.
Application : ICC/IF
Reactivity : Human

REVIEW

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Application Reference
Sun X et al. EMBO Rep 2023; 24 (9):e56230 FBL promotes cancer cell resistance to DNA damage and BRCA1 transcription via YBX1.
Bennett CL et al. Heliyon 2020; 6 (6):e04165 Tight expression regulation of senataxin, linked to motor neuron disease and ataxia, is required to avert cell-cycle block and nucleolus disassembly.
Xiangduo Kong et al. bioRxiv 2019; (Epub) The cohesin loader NIPBL interacts with pre-ribosomal RNA and treacle to regulate ribosomal RNA synthesis.
Application : ICC/IF
Reactivity : Human
Behm M et al. J Cell Sci 2017; 130 (4):745-753 Accumulation of nuclear ADAR2 regulates adenosine-to-inosine RNA editing during neuronal development.
Application : ICC/IF
Reactivity : Mouse
Zougman A et al. Nucleic Acids Res 2011; 39 (16):7103-13 Identification and characterization of a novel ubiquitous nucleolar protein 'NARR' encoded by a gene overlapping the rab34 oncogene.
Application : ICC/IF
Reactivity : Human
SDS
Sodium Azide.pdf