Fibronectin/FN, Recombinant, Human, Active

Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains fibronectin type-II domains and 16 fibronectin type-III domains. Recombinant human fibronectin fragment; is a protein of ~63kD containing a central cell-binding domain, a high affinity heparin-binding domain II and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site and a VLA-5 ligand, a cell attachment domain and virus vectors binds to a heparin binding domain II which co-locates the cell and the virus vector on NovoNectin. This process enhances the density of both cells and vectors and facilitates the gene transduction in the result.||Source:|Recombinant protein corresponding to Pro1270-Ser1546 & Ala1721-Thr2016 from human Fibronectin/FN, expressed in E. coli.||Molecular Weight: |~62.7kD||Biological Activity:|Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 0.1-0.5ug/ml.||Storage and Stability:|Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.