Images
Western Blot (Tissue lysate)
Application

Western Blot (Tissue lysate)

Western blot of rat spinal cord extracts stained with GFAP monoclonal antibody, clone 5C10 (Cat # MAB5384). A prominent band at about 50 kDa corresponds to the major isoform of GFAP.

Immunofluorescence
Application

Immunofluorescence

Mixed neuron-glial cultures stained with GFAP monoclonal antibody, clone 5C10 (Cat # MAB5384), our monoclonal antibody to GFAP (red) and chicken polylclonal antibody to neurofilament NF-L (green).
The GFAP antibody stains the network of astrocytes in these cultures, while the NF-L antibody stains neurons and their processes.
The blue channel shows the localization of DNA.

  • Specifications

    Product Description

    Mouse monoclonal antibody raised against GFAP.

    Immunogen

    Purified porcine spinal cord GFAP.

    Host

    Mouse

    Theoretical MW (kDa)

    50

    Reactivity

    Mammals, Pig

    Form

    Liquid

    Isotype

    IgG1

    Recommend Usage

    Immunocytochemistry (1:1000)
    Immunofluorescence (1:1000)
    Immunohistochemistry (1:1000)
    Western Blot (1:5000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 5 mM sodium azide).

    Storage Instruction

    Store at 4°C for short term. For long term storage, store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Tissue lysate)

    Western blot of rat spinal cord extracts stained with GFAP monoclonal antibody, clone 5C10 (Cat # MAB5384). A prominent band at about 50 kDa corresponds to the major isoform of GFAP.

    Immunohistochemistry

    Immunofluorescence

    Mixed neuron-glial cultures stained with GFAP monoclonal antibody, clone 5C10 (Cat # MAB5384), our monoclonal antibody to GFAP (red) and chicken polylclonal antibody to neurofilament NF-L (green).
    The GFAP antibody stains the network of astrocytes in these cultures, while the NF-L antibody stains neurons and their processes.
    The blue channel shows the localization of DNA.
  • Gene Info — GFAP

    Entrez GeneID

    396562

    Gene Name

    GFAP

    Gene Alias

    -

    Gene Description

    glial fibrillary acidic protein

    Gene Ontology

    Hyperlink

    Other Designations

    -

  • Publication Reference