Hepatocytes, Human in Co-Culture with Human Liver Stromal Cells

Cryopreserved adult and neonatal hepatocytes require special handling to ensure optimal viability following the freeze/thaw process. Human liver stromal cells improve metabolic and transport function as well as longevity in culture of cryopreserved Human hepatocytes beyond 40 days in culture. ||Please follow these methods closely to ensure the best performance of Human hepatocytes in co-culture with Human liver stromal Cells.||Required Equipment:|1. All procedures should be conducted inside a biological safety cabinet such as a NuAire Class II Type A2.|2. Incubator should be controlled for humidity, temperature and CO2 concentration such as a NuAire 4950 with the following settings|a. 6% CO2|b. 95% humidity|c. 37°C|Note: It is best to fyrite incubators regularly to ensure appropriate gas concentration is maintained. ||Required Supplies:|1. Fresh or cryopreserved Human hepatocytes|2. Cryopreserved growth arrested Human liver stromal cells|3. Cryopreserved Hepatocyte Recovery Media|4. Hepatocyte Plating Media|5. Hepatocyte Culture Medium|6. Collagen I coated plates |7. Liver biomatrix coated plates||Storage and Stability: |Store the complete medium at 4°C in the dark until ready for use.