HSP60 (Heat Shock Protein 60, 60kD chaperonin, 60kD heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13 (SPG13)) (PE)

Katalog-Nummer 168798-AF-PE-100ul

Size : 100ul

Marke : US Biological

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168798-AF-PE HSP60 (Heat Shock Protein 60, 60kD chaperonin, 60kD heat shock protein mitochondrial, Chaperonin, 60-KD (CPN60), GROEL, HLD4, HSP65, HSPD1, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13 (SPG13)) (PE)

Clone Type
Polyclonal
Host
mouse
Source
human
Swiss Prot
P10809 (Human); P63038 (Mouse); P63039 (Rat)
Isotype
IgG1,k
Grade
Purified
Applications
IF IHC IP WB
Crossreactivity
Ch Ec Gp Hm Hu Mk Mo Rt Ye
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-419 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp’s), more appropriately called as stress response proteins (srp’s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog. Clone LK1, unlike LK2, recognizes only the mammalian (not bacterial) hsp60 and is useful in distinguishing hsp60 from mammals and bacteria.||Applications: |Suitable for use in Immunofluorescence, Flow Cytometry (Not Tested), ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Flow Cytometry (Not Tested): 0.5-1ug/million cells|Immunofluorescence: 0.5-1ug/ml|Western Blot: 0.25-0.5ug/ml|Immunoprecipitation: 0.5-1ug/500ug protein lysate|Immunohistochemistry: Frozen only: 0.5-1ug/ml for 30 minutes at RT|Optimal dilutions to be determined by the researcher.||Positive Control:|HeLa or HepG2 cells. Synovial biopsies from patients with juvenile chronic arthritis. Synovial lining layer is strongly positive for hsp60. Breast carcinoma.||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1,k|Clone No: LK2|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with R-Phycoerythrin (PE).|Purity: Purified by Protein A/G affinity chromatography.|Immunogen: Recombinant human HSP60 protein. Cellular Localization: Mitochondria in cytoplasm|Specificity: Recognizes human HSP60. Species Crossreactivity: monkey, mouse, rat, hamster, guinea pig, chicken, E.coli, borellia, streptococcus pyogenes, yersinia entercolitica, salmonella typhimurium, treponema hyodysenteriae, treponema innocense, trichinella spiralis, helminths, yeast, and spinach. Others-not known. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Recombinant human HSP60 protein. Cellular Localization: Mitochondria in cytoplasm
Form
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human HSP60. Species Crossreactivity: monkey, mouse, rat, hamster, guinea pig, chicken, E.coli, borellia, streptococcus pyogenes, yersinia entercolitica, salmonella typhimurium, treponema hyodysenteriae, treponema innocense, trichinella spiralis, helminths, yeast, and spinach. Others-not known.
References
1. Boog CJ; de Graeff-Meeder ER; Lucassen MA; van der Zee R; Voorhorst-Ogink MM; van Kooten PJ; Geuze HJ; van Eden W. Two monoclonal antibodies generated against human hsp60 show reactivity with synovial membranes of patients with juvenile chronic arthritis. Journal of Experimental Medicine, 1992, 175(6):1805-10.