Human HMGB1/HMG-1 antibody
Katalog-Nummer AHM0915-100
Size : 100ul
Marke : NKMAX BIO
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HMGB1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1.: Jurkat cell lysate
Lane 1.: K562 cell lysate
Lane 1.: MCF7 cell lysate
Lane 1.: Jurkat cell lysate
Lane 1.: K562 cell lysate
Lane 1.: MCF7 cell lysate
Immunohistochemistry (IHC)
Paraffin embedded sections of human colon cancer tissue were incubated with anti-human HMGB1 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.