Human KLF4 protein (Recombinant)

Conjugation:	Unconjugated
Formulation:	Lyophilised from 0.2 Mu m filtered PBS solution pH 7.4.
Dilution Range:	Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction:	Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. NA
Endotoxin:	Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA

Gene Symbol:	KLF4
Gene ID:	9314
Uniprot ID:	KLF4_HUMAN
Immunogen Region:	Mature chain
Immunogen:	DNA sequence encoding mature chain of Human Krueppel-like factor 4 (KLF4) was expressed in HEK293 cells. NA

Post Translational Modifications	Ubiquitinated. 'Lys-48'-linked ubiquitinated and targeted for proteasomal degradation by the SCF(BTRC) E3 ubiquitin-protein ligase complex, thereby negatively regulating cell pluripotency maintenance and embryogenesis. Polyglutamylated by TTLL1 and TTLL4 at Glu-411, which inhibits KLF4 binding with E3 ligase component BTRC, thereby impeding ubiquitination. Deglutamylated by CCP1 and CCP6.deglutamylation promotes KLF4 ubiquitination. KLF4 glutamylation state plays a critical role in the regulation of its function in cell reprogramming, pluripotency maintenance and embryogenesis.
Function	Transcription factor.can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Protein Name	Krueppel-Like Factor 4 Epithelial Zinc Finger Protein Ezf Gut-Enriched Krueppel-Like Factor
Database Links	Reactome: R-HSA-381340 Reactome: R-HSA-422085 Reactome: R-HSA-452723 Reactome: R-HSA-9617828
Cellular Localisation	Nucleus Cytoplasm
Alternative Protein Names	Krueppel-Like Factor 4 protein Epithelial Zinc Finger Protein Ezf protein Gut-Enriched Krueppel-Like Factor protein KLF4 protein EZF protein GKLF protein

Information sourced from Uniprot.org

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