IGFBP5 ELISA Kit (Human)
Katalog-Nummer OKEH04865
Size : 96Wells
Marke : Aviva Systems Biology
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Telefonnummer : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology IGFBP5 ELISA Kit (Human) (OKEH04865) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGFBP5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IGFBP5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IGFBP5 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 78-5000pg/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-Assay CV: < 4.7% Mean Inter-Assay CV: < 7.8% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids. | ||||||||||||||||||||||
| Sensitivity | 45 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Insulin-like growth factor-binding protein 5 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | IGFBP5 |
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| Gene Full Name | insulin like growth factor binding protein 5 |
| Alias Symbols | IBP5;IBP-5;IGF-binding protein 5;IGFBP-5;insulin-like growth factor-binding protein 5. |
| NCBI Gene Id | 3488 |
| Protein Name | P24593 |
| Description of Target | Insulin-like growth factor-binding protein 5 |
| Uniprot ID | https://www.uniprot.org/uniprot/P24593 |
| Protein Accession # | NP_000590.1 |
| Nucleotide Accession # | NM_000599.3 |
| Protein Size (# AA) | 272 |
| Molecular Weight | 31 kDa |