IgG, F(ab')2 (AP)
Katalog-Nummer I1904-59X-1mg
Size : 1mg
Marke : US Biological
Contact local distributor :
Telefonnummer : +1 850 650 7790
I1904-59X IgG, F(ab')2 (AP)
Clone Type
PolyclonalHost
rabbitSource
sheepIsotype
IgGGrade
Affinity PurifiedApplications
DB E IHC WBCrossreactivity
ShShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeLabel: |Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140kD)||Applications: |Suitable for Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry. Other applications not tested. ||Recommended Dilutions: |ELISA: 1:500 to 1:2,000; using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. ||Optimal dilutions to be determined by the researcher. ||Storage and Stability:|May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: sheep|Concentration: ~0.5mg/ml (UV280)|Form: Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. |Purity: Purified by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.|Immunogen: Sheep IgG F(ab’)2 fragment|Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-rabbit serum, sheep IgG, sheep IgG F(ab’)2 and sheep serum. No reaction was observed against sheep IgG F(c).||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Sheep IgG F(ab’)2 fragment
Form
Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-rabbit serum, sheep IgG, sheep IgG F(ab’)2 and sheep serum. No reaction was observed against sheep IgG F(c).