OOSA10403 | IgG2a NEGATIVE CONTROL:RPE Negative/Isotype Control Clinisciences

Datasheets/Manuals	Printable datasheet for IgG2a NEGATIVE CONTROL:RPE Negative/Isotype Control (OOSA10403)

Product Info
Predicted Species Reactivity	Negative Control
Product Format	Lyophilized. Phosphate buffered saline
Isotype	IgG2a
Conjugation	R-PE
Application	FC
Additional Information	Buffer solution: Phosphate buffered saline pH7.4
::	Fusion Partner: Spleen cells from BALB/c mice were fused with cells from the NS1 mouse myeloma cell line. Immunogen: Activated rat T-helper cells.
::	Label Protein Molar Ratio: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant Preservative: 0.09% - Sodium Azide 1% - Bovine Serum Albumin
Reconstitution and Storage	2°C to 8°C
Specificity	This product is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue. This antibody may not be suitable for intracellular staining on some cell types. Clone MRC OX-34 recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species. This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control. Test results have shown that This product is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.
Predicted Homology Based on Immunogen Sequence	Negative Control
Immunogen	Activated rat T-helper cells.
Purification	Protein G Purified

Reference	1. Avigdor, A. et al. (2004) CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrow. Blood. 103: 2981-2989.
Description of Target	MOUSE IgG2a NEGATIVE CONTROL:RPE

IgG2a NEGATIVE CONTROL:RPE Negative/Isotype Control