IL-17A antibody (Peroxidase)
Katalog-Nummer orb345249-100ug
Size : 100ug
Marke : Biorbyt
Contact local distributor :
Telefonnummer : +1 850 650 7790
IL-17A antibody (Peroxidase)
Catalog Number: orb345249
| Catalog Number | orb345249 |
|---|---|
| Category | Antibodies |
| Description | IL-17A antibody (Peroxidase) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC, WB |
| Reactivity | Mouse, Rat |
| Isotype | IgG |
| Immunogen | This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant rat IL17-A protein. |
| Concentration | 1.0 mg/mL |
| Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000 |
| Form/Appearance | Lyophilized |
| Purity | This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant rat IL17-A in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended. |
| Conjugation | HRP |
| UniProt ID | Q61453 |
| NCBI | Q61453.1 |
| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Buffer/Preservatives | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Alternative names | rabbit anti-IL-17A Peroxidase Conjugated Antibody, |
| Note | For research use only |
| Application notes | This purified antibody has been tested for use in western blotting and suitable in ELISA. By western blot a band approximately 30.0 kDa in size corresponding to rat IL17-A protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. |
| Expiration Date | 12 months from date of receipt. |
Western Blot of Rabbit anti-Rat IL-17A Antibody Peroxidase Conjugated. Lane 1: Recombinant Rat IL-17A. Load: 50 ng per lane. Primary antibody: Rabbit anti-Rat IL-17A Antibody Peroxidase Conjugated at 1:1000 o/n at 4°C. Secondary antibody: none. Block: orb348637 for 30 minutes at RT. Predicted/Observed size: 17 kDa, 17 kDa for Rat IL-17A.