Sample Type:|Serum, plasma, tissue homogenates and other biological fluids.||Intended Use:|Sandwich ELISA, Double Antibody||Sensitivity:|<9.375pg/ml||Range:|15.625-1000pg/ml||Specificity:|Canine||Test Principle:|This BioAssay™ kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-1beta antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-1beta antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-1beta amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-1beta can be calculated.||Kit Components:|Microtiter Strips, 1x48 wells or 1x96 wells |Standard, 1x1 vial or 2x1 vials|Sample/Standard Dilution Buffer, 1x10ml or 1x20ml|Antibody (Biotin) (Concentrated), 1x30ul or 1x60ul|Antibody Dilution Buffer, 1x5ml or 1x10ml|Streptavidin (HRP) (SABC), 1x60ul or 1x120ul|SABC Dilution Buffer, 1x5ml or 1x10ml|TMB Substrate, 1x5ml or 1x10ml|Stop Solution, 1x5ml or 1x10ml|Wash Buffer, 25X, 1x15ml or 1x30ml||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.