INF2 (Inverted Formin-2, HBEBP2-binding Protein C, C14orf151, C14orf173) (APC)

NF2 (inverted formin 2) is a mouse formin that has been found to interact with actin through the C-terminus to the formin homology 2 domain. The WASP homology 2 motif activates another region that appropriates actin monomers. INF2 has a unique ability to speed up polymerization and depolymerization of actin filaments. Mutations in the N-terminal diaphanous inhibitory domain of INF2 have been proven to be a main cause of familial focal segmental glomerulosclerosis.||Applications:|Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.||Recommended Dilutions:|Immunohistochemistry: Requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer, pH 6.0 is recommended.|Optimal dilutions to be determined by the researcher.||Positive Control:|HeLa cell lysate||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.