ASM10556-A565-100 | Kir4.1 Antibody Clinisciences

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Kir4.1 Polyclonal Antibody (ASM10556). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Kir4.1 Polyclonal Antibody (ASM10556) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane, Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Kir4.1 Antibody. (D) Composite.
Western blot analysis of Rat Liver cell lysates showing detection of ~42.5 kDa Kir4.1 protein using Rabbit Anti-Kir4.1 Polyclonal Antibody (ASM10556). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Kir4.1 Polyclonal Antibody (ASM10556) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~42.5 kDa.

Product Information
Application Applications Legend: WB=Western Blot IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin-embedded Sections) IHC-F=Immunohistochemistry (Frozen Sections) IF=Immunofluorescence FC=Flow Cytopmetry IC=Immunochemistry ICC=Immunocytochemistry E=ELISA IP=Immunoprecipitation DB=Dot Blot CHIP=Chromatin Immunoprecipitation FA=Fluorescence Assay IEM=Immuno electron microscopy EIA=Enzyme Immunoassay	WB, ICC
Primary Accession	P78508
Other Accession	NP_002232.2
Host	Rabbit
Reactivity	Human
Clonality	Polyclonal
Description	Rabbit Anti-Human Kir4.1 Polyclonal
Target/Specificity	Detects ~42 kDa.
Other Names	ATP sensitive inward rectifier potassium channel 10 Antibody, BIRK10 Antibody, KCNJ13 PEN Antibody, Potassium channel inwardly rectifying subfamily J member 10 Antibody, SESAME Antibody inwardly rectifying subfamily J member 10 Antibody, Inward rectifier K(+) channel Kir1.2 Antibody, Potassium channel KCNJ 10 Antibody, ATP-sensitive inward rectifier potassium channel 10 Antibody, Potassium inwardly rectifying channel subfamily J member 10 Antibody, KIR1.2 Antibody, Inwardly rectifying potassium channel Kir1.2 Antibody, ATP-dependent inwardly rectifying potassium channel Kir4.1 Antibody, Glial ATP dependent inwardly rectifying potassium channel KIR4.1 Antibody, Kcnj10 Antibody, KCNJ10 Antibody, IRK10_HUMAN Antibody, KIR4.1 Antibody, Inward rectifier K+ channel KIR1.2 Antibody, ATP dependent inwardly rectifying potassium channel Kir4.1 Antibody, Potassium channel Antibody
Immunogen	Synthetic peptide from the mid-protein of Human Kir4.1
Purification	Peptide Affinity Purified
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature	Blue Ice or 4ºC
Certificate of Analysis	A 1:1000 dilution of SPC-700 was sufficient for detection of Kir4.1 in 15 µg of rat liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization	Membrane \| Multi-Pass Membrane Protein \| Basolateral Cell Membrane

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