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Western Blot (Transfected lysate)
The cell lysate derived from conditioned LRP5 transfected HEK293 was immunoprobed by LRP5 (phospho T1492) polyclonal antibody (Cat # PAB12631) at 1:500 dilution. An immunoreactive band is observed around ~175kDa (lane 1). This band is abolished by pre-incubation with immunizing peptide (lane 2).
Dot Blot (Peptide)
1 µg peptide was blot onto NC membrane (A: LRP5 (pT1492); B: LRP5 (non-phospho); C: Non-related phosphopeptide) was blotted by LRP5 (phospho T1492) polyclonal antibody (Cat # PAB12631) at 1:2000 dilution.
- Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of LRP5.
Immunogen
Synthetic phosphopeptide corresponding to epitope KATLY surrounding T1492 of human LRP5.
Host
Rabbit
Theoretical MW (kDa)
175
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. - Applications
Western Blot (Transfected lysate)
The cell lysate derived from conditioned LRP5 transfected HEK293 was immunoprobed by LRP5 (phospho T1492) polyclonal antibody (Cat # PAB12631) at 1:500 dilution. An immunoreactive band is observed around ~175kDa (lane 1). This band is abolished by pre-incubation with immunizing peptide (lane 2).Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Dot Blot (Peptide)
1 µg peptide was blot onto NC membrane (A: LRP5 (pT1492); B: LRP5 (non-phospho); C: Non-related phosphopeptide) was blotted by LRP5 (phospho T1492) polyclonal antibody (Cat # PAB12631) at 1:2000 dilution. - Gene Info — LRP5
Entrez GeneID
4041Gene Name
LRP5
Gene Alias
BMND1, EVR1, EVR4, HBM, LR3, LRP7, OPPG, OPS, OPTA1, VBCH2
Gene Description
low density lipoprotein receptor-related protein 5
Gene Ontology
HyperlinkOther Designations
exudative vitreoretinopathy 1|low density lipoprotein receptor-related protein 7|osteoporosis pseudoglioma syndrome
- Interactomes
- Pathways
- Diseases
- Publication Reference
- Aberrantly activated Wnt/β-catenin pathway co-receptors LRP5 and LRP6 regulate osteoblast differentiation in the developing coronal sutures of an Apert syndrome (Fgfr2 S252W /+ ) mouse model.
Nay Myo Min Swe, Yukiho Kobayashi, Hiroyuki Kamimoto, Keiji Moriyama.
Developmental Dynamics 2021 Mar; 250(3):465.
Application：IHC, Mouse, Mouse osteogenic fronts.
- LGR4 acts as a key receptor for R-spondin 2 to promote osteogenesis through Wnt signaling pathway.
Zhu C, Zheng XF, Yang YH, Li B, Wang YR, Jiang SD, Jiang LS.
Cellular Signalling 2016 Aug; 28(8):989.
Application：WB-Ce, WB-Tr, Mouse, MC3T3-E1 cells.
- Astragaloside IV Inhibits the Up-Regulation of Wnt/β-Catenin Signaling in Rats with Unilateral Ureteral Obstruction.
Wang L, Chi YF, Yuan ZT, Zhou WC, Yin PH, Zhang XM, Peng W.
Cellular Physiology and Biochemistry 2014 Apr; 33(5):1316.
Application：WB-Ti, Rat, Kidney.
- Casein kinase 1 gamma couples Wnt receptor activation to cytoplasmic signal transduction.
Davidson G, Wu W, Shen J, Bilic J, Fenger U, Stannek P, Glinka A, Niehrs C.
Nature 2005 Dec; 438(7069):867.
- Aberrantly activated Wnt/β-catenin pathway co-receptors LRP5 and LRP6 regulate osteoblast differentiation in the developing coronal sutures of an Apert syndrome (Fgfr2 S252W /+ ) mouse model.