LTA recombinant monoclonal antibody, clone 3F12.2D3

Western Blot

Western blot analysis of lipopolysaccharide(LPS)-treated human peripheral blood monocyte (PBM) with LTA recombinant monoclonal antibody, clone 3F12.2D3 (Cat # RAB03637).
Western Blot analysis the LPS-treated human PBM lysates (35ug protein in RIPA buffer) were resolved on a 15% SDS PAGE gel and blots were probed with the chimeric r version of RAB03637 at 2 ug/mL before detection using an anti-rondary antibody. Protein was detected by chemiluminescence.

Enzyme-linked Immunoabsorbent Assay

Flow Cytometry

Flow cytometric analysis of U937 cells with LTA recombinant monoclonal antibody, clone 3F12.2D3 (Cat # RAB03637).
U937 cells were fixed using 2% PFA and permeabilized with 0.5% Triton and stained with unimmunized r antibody (isotype control-black line) or the r chimeric version of RAB03637 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Neutralization

Surface Plasmon Resonance

Entrez GeneID

4049

Gene Name

LTA

Gene Alias

LT, TNFB, TNFSF1

Gene Description

lymphotoxin alpha (TNF superfamily, member 1)

Omim ID

153440

Gene Ontology

Hyperlink

Gene Summary

The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4 and psoriatic arthritis

Other Designations

OTTHUMP00000029184|OTTHUMP00000037612|OTTHUMP00000037613|OTTHUMP00000165897|lymphotoxin alpha|tumor necrosis factor beta