Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen) (BSA & Azide Free) (HRP)

L1 Protein or Calprotectin Molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages.||Applications:|Suitable for use in Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Immunohistochemistry (Formalin/paraffin): 1:1000 for 20 minutes at RT. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml in PBS for 10 minutes at 37°C.|Optimal dilutions to be determined by the researcher.||Positive Control: |Tonsil, lymph node or spleen||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.     ||Note: Applications are based on unconjugated antibody.