MMP7 ELISA Kit (Mouse)

Katalog-Nummer OKEH00183

Size : 96Wells

Marke : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00183
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology MMP7 ELISA Kit (Mouse) (OKEH00183) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP7 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.2% (n = 20)
Mean Inter-assay CV%: < 9.8% (n = 20)
ELISA Kit Component
ComponentAmount
MMP7 Microplate96 Wells (12 x 8 Well strips)
MMP7 Lyophilized Standard2
100X Biotinylated MMP7 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity41.1 pg/mL
SpecificityNatural and recombinant Mouse Matrilysin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolMMP7
Gene Full Namematrix metallopeptidase 7
Alias SymbolsMatrin;Matrix metalloproteinase-7;Pump-1 protease;Uterine metalloproteinase.
NCBI Gene Id17393
Protein NameQ10738
Description of TargetMatrilysin
Uniprot IDhttps://www.uniprot.org/uniprot/Q10738
Protein Accession #NP_001306915
Nucleotide Accession #NM_001319986
Protein Size (# AA)264
Molecular Weight30 kDa

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Katalog-Nummer
Beschreibung
Cond.
Preis zzgl. MwSt.
OKEH03339
 96Wells