Conjugation | APC-iFluor™ 700 |
---|---|
Antigen | CD4 |
Clone | SK3 |
Isotype | IgG1, k |
Applications | FC |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Primary/Secondary | Primary |
Immunogen | Human peripheral blood T lymphocytes |
Format | 0.09% sodium azide, 0.2% BSA (w/v), PBS pH 7.2 |
Storage | 2-8°C |
Status | RUO |
Excitation Laser | Red (638nm) |
CD4 APC-iFluor™ 700
Catalog #
Size
Description:
The clone SK3, a mouse monoclonal antibody selectively binds with a 55-59kD type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD4 contains four extracellular Ig-like domains (D1-D4). The epitope for SK3 is located within the D3 domain of the protein, which has a structure resembling an Ig variable domain. Expression of CD4 is observed in subsets of T lymphocytes, monocytes, macrophages, and dendritic cells. Through interaction of MHC-II, CD4 facilitates cell-cell interaction, thymic differentiation, and activation of downstream signaling cascades. HIV infection of T-cells is instigated through binding of HIV to CD4. Binding of SK3 antibody blocks HIV infection through CD4 and also blocks the mixed lymphocyte reaction (MLR).
The clone SK3, a mouse monoclonal antibody selectively binds with a 55-59kD type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD4 contains four extracellular Ig-like domains (D1-D4). The epitope for SK3 is located within the D3 domain of the protein, which has a structure resembling an Ig variable domain. Expression of CD4 is observed in subsets of T lymphocytes, monocytes, macrophages, and dendritic cells. Through interaction of MHC-II, CD4 facilitates cell-cell interaction, thymic differentiation, and activation of downstream signaling cascades. HIV infection of T-cells is instigated through binding of HIV to CD4. Binding of SK3 antibody blocks HIV infection through CD4 and also blocks the mixed lymphocyte reaction (MLR).