General Info
| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.065ng/mL |
| Detection Limit: | 0.156-10ng/mL |
| Short Description: | This ENPP2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
Information
| Gene Symbol: | Enpp2 |
| Gene ID: | 18606 |
| Uniprot ID: | ENPP2_MOUSE |
| Sample Type: | serum, plasma, tissue homogenates or other biological fluids. |
Description
| Tissue Specificity | Expressed in brain and adipose tissue. |
| Post Translational Modifications | N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity. Secretion requires simultaneous glycosylation on Asn-53 and Asn-410, while probable glycosylation of Asn-410 has a preferential role on lysoPLD activity. Not O-glycosylated. The interdomain disulfide bond between Cys-413 and Cys-805 is essential for catalytic activity. |
| Function | Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development (Probable). Tumor cell motility-stimulating factor. Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids. Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF). |
| Protein Name | Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2E-Npp 2AutotaxinExtracellular Lysophospholipase DLysopld |
| Database Links | |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2 ELISA kitE-Npp 2 ELISA kitAutotaxin ELISA kitExtracellular Lysophospholipase D ELISA kitLysopld ELISA kitEnpp2 ELISA kitNpps2 ELISA kitPdnp2 ELISA kit |
| output |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance