Mouse IgG Fab monoclonal antibody, clone RMG05 (Biotin)
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- More Files
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgG Fab monoclonal antibody, clone RMG05 (Cat # MAB12775) at the following concentrations: 0.5 ug/mL, 0.2 ug/mL, and 0.05 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fab region of mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.
- Specifications
Product Description
Goat recombinant monoclonal antibody raised against mouse IgG Fab.
Antibody Species
Goat
Immunogen
Original antibody is raised against mouse IgG.
Sequence
N/A
Reactivity
Mouse
Form
Liquid
Conjugation
Biotin
Purification
Protein G purification
Isotype
IgG
Recommend Usage
ELISA (0.05-1 ug/mL)
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (0.5-2 ug/mL)
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Mouse IgG Fab monoclonal antibody, clone RMG05 (Cat # MAB12775) at the following concentrations: 0.5 ug/mL, 0.2 ug/mL, and 0.05 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to the Fab region of mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. An alkaline phosphatase conjugated anti-goat IgG as the secondary antibody.Flow Cytometry