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> Mouse IgG3 antibody (ATTO 550)

Mouse IgG3 antibody (ATTO 550)

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Katalog-Nummer orb347462-500ug

Size : 500ug

Marke : Biorbyt

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    • Mouse IgG3 antibody (ATTO 550) [Out of stock]

    Mouse IgG3 antibody (ATTO 550) [Out of stock]

    Catalog Number: orb347462

    Catalog Numberorb347462
    CategoryAntibodies
    DescriptionMouse IgG3 antibody (ATTO 550)
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsFC, FLISA, IF, IHC, WB
    ReactivityMouse
    IsotypeIgG
    ImmunogenMouse IgG3 heavy chain
    Concentration1.0 mg/mL
    Dilution rangeFLISA: >1:20,000, FC: 1:500 - 1:2,500, IHC: >1:5,000, IF: >1:5,000, WB: >1:10,000
    Form/AppearanceLyophilized
    PurityAnti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
    ConjugationATTO-550
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesgoat anti-MOUSE IgG3 Antibody ATTO550 Conjugation,
    NoteFor research use only
    Application notesATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Anti-Mouse IgG3 secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG3 antibody based assays. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
    Expiration Date12 months from date of receipt.
    Mouse IgG3 antibody (ATTO 550) [Out of stock]

    ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.

    Mouse IgG3 antibody (ATTO 550) [Out of stock]

    Atto™ dyes can be used for multicolor immunofluorescent detection with low background and high signal. Example shown here is Immunohistochemical staining using ATTO-550 Anti-Aquaporin 2-antibody (red) of paraffin embedded region of rat kidney showing a transversal cut of the inner medulla near to the renal papilla. Nuclei are visualized with Hoechst 33342 (blue).