Mycoplasma pneumoniae IgM ELISA Kit
Katalog-Nummer EA101078
Size : 1kit
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Mycoplasma pneumoniae IgM ELISA Kit
| Product Data | |
| Assay Length | 3 hours |
|---|---|
| Assay Type | Solid Phase Sandwich ELISA |
| Assay Type | Sandwich |
| Background | The Mycoplasma pneumoniae IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Specimen samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false results. Microtiter wells as a solid phase are coated with Mycoplasma pneumoniae antigen. Pretreated sample specimens and ready-for-use controls are pipetted into these wells. During incubation Mycoplasma pneumoniae-specific antibodies of high specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of high results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Mycoplasma pneumoniae-specific IgM antibody in the sample specimen. Absorbance at 450 nm is read using an ELISA microtiter plate reader. |
| Components | See the product information for more details |
| Format | 96-well strip plate |
| Interference | No significant interference observed with available related molecules. |
| Reactivity | Human |
| Reactivity | Human |
| Reactivity | Human |
| Sample Type | Cell culture supernatant, serum, plasma |
| Sample Volume | 10 ul |
| Signal | Colorimetric |
| Species | Human |
| Specificity | Natural and recombinant human Mycoplasma pneumoniae IgM |
| New | No |
| Description | Mycoplasma pneumoniae IgM ELISA Kit |