Mynox® Gold is a further development of the classic Mynox®. Mynox® Gold is a combination of a standard antibiotic ciprofloxacin with the biological Mynox® reagent surfactin. One application includes an initial treatment that eliminates most mycoplasmas without affecting the cells. The follow-up treatments destroy the remaining mycoplasmas. The mode of action of Mynox® Gold is based on a biophysical mechanism to integrate into the membrane of mycoplasmas and thereby impair their integrity.

Benefits

• Functionality test

  Technical Note on the functional testing of Mynox® Gold. Removal of mycoplasma from a Vero cell line contaminated with mycoplasma.

• Low resistance risk

  The combination of the standard antibiotic ciprofloxacin and the Mynox® reagent surfactin virtually eliminates the possibility of resistance to Mynox® Gold when eliminating mycoplasmas.

• Highly effective

  The eradication efficiency is close to 100 %.

• Mycoplasma removal from virus stocks

  Protocol for the pre-treatment of virus stocks available as a Technical Note.

Recommended Use

Mynox® Gold is intended for research use only and not for clinical applications. Not applicable for the direct treatment of cells that serve as host cells for Chlamydia or other bacterial host cells. The level of antibiotics in the product may affect the integrity of the parasites.

Content

One application of Mynox® Gold contains one vial for the starter treatment and three vials for the main treatment. Each vial contains 500 µl as a sterile, ready-to-use solution.

Package Sizes

A single application is sufficient to permanently remove mycoplasma from a cell culture.

• Cat. No. 10-0201 2 applications
• Cat. No. 10-0501 5 applications
• Cat. No. 10-1001 10 applications

Result Evaluation

Elimination success should be verified with a suitable mycoplasma detection system, e.g. Minerva Biolabs' Venor®GeM Mycoplasma PCR Detection Kits.

Required Consumables

25 cm² cell culture flasks or tissue culture petri dishes (60 mm Ø)
Standard cell culture plasticware

Required Lab Devices

Standard cell culture equipment (incubator, centrifuge, pipettes, etc.)

Shelf Life and Storage

Store the components at +2 °C to +8 °C until the expiry date indicated on the label. Protect from light.

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