Oil Red O

Biological Activity

Oil red O (ORO) is a common dye for tissue staining that recognizes nitroglycerin and cholesteryl esters. Oil red O encounters dsDNA, which can fluoresce when the emission peak is at 600 nm.

Chemical Information

Molecular Weight	408.49
Formula	C26H24N4O
CAS Number	1320-06-5
Solubility (25°C)	DMSO ≥ 5 mg/mL
Storage	-20°C, protect from light, sealed

References

[1] Yang Liu, et al. Talanta . New role of oil red O in detection of double stranded DNA

[2] Feng-Yung Wang, et al. Bio Protoc. Oil Red O Staining for Lipid Content in Caenorhabditis elegans

[3] Wilber Escorcia, et al. J Vis Exp. Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining

[4] M Jess Andrs-Manzano, et al. Methods Mol Biol. Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root

[5] Manuel J Deutsch, et al. Anal Biochem. Digital image analysis approach for lipid droplet size quantitation of Oil Red O-stained cultured cells

[6] Annika Mehlem, et al. Nat Protoc. Imaging of neutral lipids by oil red O for analyzing the metabolic status in health and disease