PCR SuperMix (-dye)

PCR SuperMix is a ready-to-use mixture of DNA Polymerase, dNTPs and optimized buffer. The supermix is provided at 2x concentration and used at 1x concentration by adding the template, primers and nuclease-free water. PCR products are unsuitable for PAGE. The Extension rate is about 1-2 kb/min. Amplification of genomic DNA fragment up to 4 kb. Contents:

Component	1 ml size	5 ml size	15 ml size
2X PCR SuperMix (-dye)	1 ml	5 × 1 ml	15 × 1 ml
ddH2O	1 ml	5 ml	3 × 5 ml

Target	PCR SuperMix (-dye)
Storage	Store at -20°C. Stable for 12 months from date of receipt.
Availability	Shipped within 10-20 working days.
Note	This product is for research use only.
Directions for use	Reaction Components: Component Volume Final Concentration Template Variable as required Forward Primer (10 µM) 1 µl 0.2 µM Reverse Primer (10 µM) 1 µl 0.2 µM 2X PCR SuperMix 25 µl 1X ddH2O Variable N/A Total Volume 50 µl N/A Thermal Cycling Conditions: 94 °C, 2-5 min 94 °C, 20 s 50-60 °C, 20 s 72 °C, 2-6 kb/min Repeat 2-4 for 30-35 cycles. 72 °C, 5-10 min Notes: Before use, completely thaw all components and mix well.

Research Articles on PCR SuperMix (-dye)