pEASY®-T1 Cloning Kit
Katalog-Nummer CT101-01
Size : 20rxns
Marke : TransGen Biotech
Contact local distributor :
Telefonnummer : +1 850 650 7790
ManualpEASY®-T1 Cloning Kit is designed for cloning and sequencing Taq-amplified PCR products.
•5 minutes fast ligation of Taq-amplified PCR products.
•Kanamycin and Ampicillin resistance genes for selection.
•Easy blue/white selection.
•T7 promoter, M13 forward and M13 reverse primers for sequencing.
•T7 promoter for in vitro transcription.
•Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
Storage
Competent Cell at -70°C for six months; others at -20°C for 9 months
Shipping
Dry ice (-70℃)
| Component | CT101-01 (20 rxns) | CT101-02 (60 rxns) |
| pEASY ®-T1 Cloning Vector (10 ng/μl) | 20 μl | 3×20 μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cells | 10×100 μl | 30×100 μl |
Zuo W , Chao Q , Zhang N , et al. A maize wall-associated kinase confers quantitative resistance to head smut[J]. Nature Genetics, 2015, 47(2):151-157.
Sun W , Shen Y H , Han M J , et al. An Adaptive Transposable Element Insertion in the Regulatory Region of the EO Gene in the Domesticated Silkworm, Bombyx mori[J]. Molecular Biology and Evolution, 2014, 31(12):3302-3313.
Xiang X , Huang X , Wang H , et al. pTC Plasmids from Sulfolobus Species in the Geothermal Area of Tengchong, China: Genomic Conservation and Naturally-Occurring Variations as a Result of Transposition by Mobile Genetic Elements[J]. Life, 2015, 5(1):506-520.
Guo Z , Kang S , Zhu X , et al. The novel ABC transporter ABCH1 is a potential target for RNAi-based insect pest control and resistance management[J]. Scientific Reports, 2015, 5(1):13728.
Wang X , He G , Mai K , et al. Differential regulation of taurine biosynthesis in rainbow trout and Japanese flounder[J]. Scientific Reports, 2016, 6:21231.