PerfectStart^® III Fast Probe qPCR SuperMix UDG is supplied at a 2× concentration, and is a pre-mix reagent for the amplification and detection of DNA in probe-based qPCR. Simply add primers, probe, template, and Nuclease-free Water to perform singleplex, multiplex (up to quadruple) PCR reactions in one reaction well. The amount of fluorescence is proportional to the amount of amplified product during amplification, so the amount of nucleic acid in the sample can be determined by the detection of the amount of fluorescence. PerfectStart^® Taq III Hot-Start DNA Polymerase(Genetically modified Taq DNA polymerase, using antibody blocking method to effectively block the activity of DNA polymerase to prevent non-specific amplification at low temperature), combined with optimal reaction buffer, which significantly improves template affinity, qPCR amplification performance and the sensitivity of low-concentration template detection. It supports full premixing of primers and probes, and has good impurity tolerance to low-purity nucleic acids (presence of inhibitors), which is suitable for a variety of detection scene. The dUTP/UDG in the SuperMix can function at room temperature to eliminate carry-over contamination caused by PCR products and ensure the accuracy of results.

Features 

• Hot start Taq DNA Polymerase blocking by antibodies, enabling high specificity, high sensitivity, high amplification efficiency, and a wide range of applicable species. 

• Specially optimized qPCR reaction buffer provides higher sensitivity and specificity. 

• High-efficiency antibody blocking, with optimal qPCR reaction buffer, supports full premixing of primers and probes, and enhances anti-inhibition. 

• The dUTP/UDG anti-contamination system is used to effectively prevent carry-over contamination of PCR products, ensuring the accuracy of results. 

• Universal Passive Reference Dye for different instruments to correct differences in fluorescence detection between wells due to pipetting errors.

Applications

Detection of DNA of animal, plant and microorganism by probe fluorescence quantitative PCR

Storage

at -20℃ for two years

Shipping

Dry ice (-70℃)