pgp9.5 (UCHL-1)(31A3), 0.2mg/mL

Katalog-Nummer BNUB0046-100

Size : 100uL

Marke : Biotium

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Antibody number

#0046

Antibody reactivity (target)

PGP9.5, UchL1

Antibody type

Primary

Host species

Mouse

Clonality

Monoclonal

Clone

31A3

Isotype

IgG1, kappa

Molecular weight

20-30 kDa

Synonyms

Gracile Axonal Dystrophy, Neuron Cytoplasmic Protein 9.5, Park5, Parkinson Disease 5, PGP95, Protein Gene Product 9.5, Ubiquitin Carboxyl-terminal Esterase L1, Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1, Ubiquitin Thioesterase L1, Ubiquitin Thiolesterase L1

Human gene symbol

UCHL1

Entrez gene ID

7345

SwissProt

P09936

Unigene

518731

Immunogen

Native UchL1 (PGP9.5) protein from brain

Verified antibody applications

Flow (intracellular) (verified), IF (verified), IHC (FFPE) (verified), WB (verified)

Antibody target cellular localization

Cytoplasmic, Endoplasmic reticulum

Species reactivity

Cow, Human, Mouse, Pig, Rat

Antibody application notes

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunohistology formalin-fixed 0.5-1 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes, Western blotting 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user

Positive control

Cerebellum

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide

Antibody research areas

Metabolism, Neuroscience

Product origin

Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.

Cell/tissue expression

Neurons

Protocols
SDS
Supporting Documents

Download Datasheet

Product Description

This MAb reacts with a protein of 20-30 kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson’s disease.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the

CF® Dye Brochure

for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email

info@clinisciences.com

to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 0046, Anti-PGP9.5 (31A3) 

Antibody # prefix Conjugation Ex/Em (nm) Laser line Detection channel Dye Features
BNC04 CF®405S 404/431 405 DAPI (microscopy), AF405

CF®405S Features

BNC88 CF®488A 490/515 488 GFP, FITC

CF®488A Features

BNC68 CF®568 562/583 532, 561 RFP, TRITC

CF®568 Features

BNC94 CF®594 593/614 561 Texas Red®

CF®594 Features

BNC40 CF®640R 642/662 633-640 Cy®5

CF®640R Features

BNC47 CF®647 650/665 633-640 Cy®5

CF®647 Features

BNC74 CF®740 742/767 633-685 775/50

CF®740 Features

BNCB Biotin N/A N/A N/A
BNUB Purified N/A N/A N/A
BNUM Purified,
BSA-free
N/A N/A N/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.